Conjugation

Part:BBa_K4761030

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-29)


linker1

The linker gene 1 used for adh6-ARO10 fusion expression. Fusion expression of enzymes corresponding to two adjacent reactions in the metabolic pathway may have an impact on the reaction rate. In addition, the type of connector also affects the fusion expression effect[1]. The sequence of Linker1 is: GGCGGCGGCGGCTCC The amino acids encoded are: (Gly-Gly-Gly-Gly-Ser) It is a flexible joint.

[1]Xiaoying Chen, Jennica L. Zaro, Wei-Chiang Shen,Fusion protein linkers: Property, design and functionality,Advanced Drug Delivery Reviews,Volume 65, Issue 10,2013,Pages 1357-1369,ISSN 0169-409X,https://doi.org/10.1016/j.addr.2012.09.039.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None